The newest product from PNOĒ, a company based in Malden, Massachusetts, with operations in Athens, Greece, looks like a mask worn by Bane, a character from the Batman comics, but it is designed to measure oxygen consumption and carbon dioxide exhalation. This data can then be used to provide advice on diet, training, and longevity. The PNOĒ 2.0 is set to launch on October 1 and allows users to perform the test themselves, unlike the current version, which requires a trained operator. According to co-founder and CEO Apostolos Atsalakis, users can simply wear the mask, press a button, and breathe for eight minutes. The device is smaller and more compact than its predecessor, with fewer parts, and was designed with Milan-based Design Group Italia after many iterations. The electronics detach from the silicone mask and straps, allowing multiple users to share the hardware while keeping their own masks. The science behind PNOĒ is not new; metabolic testing has been around for over a century and is the gold standard for measuring VO₂ max, a key indicator of cardiorespiratory fitness. However, traditional tests require bulky and expensive equipment found mainly in sports labs and hospitals. PNOĒ aims to make the same accuracy available in a portable package, paired with software that translates results into recommendations. The device captures 23 biomarkers, including resting metabolic rate and metabolic flexibility. Atsalakis argues these metrics answer questions that blood tests cannot, such as how many calories a person needs or how they should train. VO₂ max has become a buzzword among longevity enthusiasts, with research linking higher cardiorespiratory fitness to lower mortality. Atsalakis calls VO₂ max the strongest predictor of human longevity and sees his company’s data as a scorecard for the wellness industry. The device is not cleared by the U.S. Food and Drug Administration, and Atsalakis said the company does not provide medical recommendations, considering it a wellness device rather than a medical tool. PNOĒ traces its roots to Atsalakis's PhD work in sensing technologies at the University of Cambridge. He co-founded the company with Panos Papadiamantis, a childhood friend who is now the company’s chief product officer. The startup went through Y Combinator’s Winter 2019 batch and has raised about $22 million, including a recently closed $11 million round from investors Fifty Years and Google Maps co-founder Lars Rasmussen. PNOĒ sells only to businesses, which then offer the test to their customers. Clients include Equinox, Four Seasons hotels, Red Bull, the NBA, the Mount Sinai Health System, and the med spa chain Restore Hyper Wellness. About 85% of its business comes from the U.S., which Atsalakis described as "by far the most advanced market globally" for longevity. The company is now expanding in Europe and, through partners, in Latin America and Asia. Businesses pay a subscription ranging from $400 a month to more than $1,000, which covers the hardware, software, training, and marketing materials. PNOĒ also links its results to the services a business sells, so a gym or spa can recommend specific offerings based on a customer’s test. Many clients use the test during onboarding, positioning it somewhere between a full clinical workup and the estimates people get from their smartwatches. The market for such devices is increasingly crowded, with companies like Apple, Garmin, and Whoop estimating VO₂ max from heart-rate data, while consumer devices like Lumen analyze breath to gauge fat and carb burning. Traditional metabolic carts remain the standard in labs and hospitals. PNOĒ, which employs 110 people, says it recently turned profitable, while growing more than 100% a year. Atsalakis said the company plans to raise a Series B within the next six to 12 months as it tries to put its mask — Bane comparisons and all — in front of more faces.